Is it possible to analyse audio signals at the audio output (e.g. coming from a media player software)?
On Windows systems you will find “Loopback Output” devices in the “Windows Audio” submenu, that can be selected as inputs. This loopback is only available if your player software uses a Windows Audio driver as output. When playing back audio from a DAW software, which normally uses ASIO drivers, you can use the IQ-Series Analyser NetAux plugin to show a signal in IQ-Series Analyser (Standalone) (see chapter Network function in the manual for details).
Unfortunately, on OS X systems a direct loopback of the audio output is not possible. You need to use an additional audio routing tool to achieve this. For instance, you could use Soundflower (https://rogueamoeba.com/freebies/soundflower/) which is available for free. Of course you can also use the IQ-Series Analyser NetAux plugin with a DAW on OS X.